Assignment 4

Finding the desired recombinant DNA involves several selection steps, one of which is growing transformed cells on antibiotic containing media. A selection step is any step that allows us to remove undesired recombinants from the pool of DNA ligation products. Describe all the selection steps we are using to find the desired recombinant plasmid and for each step explain what DNA is selected out.

This assignment must be submitted via turnitin.com.

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