Assignment 5

Identifying the desired recombinant(s).

One of our goals is to produce a new recombinant plasmid that includes GeneA in its multiple cloning site. Unfortunately, it is easy to make recombinants that are not desirable (for example, recombinants that have an insert that we don't want or no insert at all). In order to identify our desired recombinant, we employed several selection steps. Briefly describe these selection steps and for each step, explain which undesirable DNA molecules would be screened out.

Your answer should be less than one page long.

Assignment must be submitted via turn-it-in

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