Assignment 4 - 2015
  1. For both the targeted and the shotgun cloning methods, diagram all possible 2 piece recombinant molecules. Label relevant restriction enzyme sites, the origins of replication, the antibiotic resistance genes, and the location of Gene A if present.
  2. For each of the above recombinants, indicate if the bacteria that take it up will
    • be able to replicate the plasmid (indicate as 'replicatable')
    • grow on ampicillin containing plates (ampR) and
    • if the resulting colonies will be blue or white on x-gal containing plates (blue or white).

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