Assignment 3 - 2016

Vector features

This assignment must be submitted via turn-it-in

One goal of this project is to subclone GeneA from the pKC7 plasmid (pBR322 plasmid with GeneA inserted) into the pUC19 vector.

  1. pKC7 is a derivative of the pBR322 vector. Gene A was inserted into pBR322 using the restriction enzymes BamHI and HindIII. pBR322 is resistant to both tetracycline and ampicillin but pKC7 is only resistant to ampicillin. Explain why pKC7 is no longer tetracycline resistant. Hint: look carefully at the plasmid map for pBR322. (One to two sentences)
  2. The pUC18 vector is a derivative of pBR322. How does the pUC18 vector differ from the pBR322 vector? Note that pUC18 and pUC19 are essentially the same vector so anything you find on pUC19 is true for pUC18.
  3. Describe three good reasons why pUC18 is generally a more popular vector than pBR322.

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