Assignment 4 - 2017
  1. For both the targeted and the shotgun cloning methods, draw plasmid maps of all possible 2 piece recombinant molecules.
    • Indicate the total size of each recombinant and the source and size of each fragment (colour coding is fine).
    • Indicate the relevant restriction enzyme sites (abbreviations are fine, just give us a key), the origins of replication, the antibiotic resistance genes, and the location of Gene A, if present.
  2. For each of the above recombinants indicate if the bacteria that take it up will
    • be able to replicate the plasmid (indicate as 'replicatable')
    • grow on ampicillin-containing plates (ampR) and
    • if the resulting colonies will be blue or white on x-gal containing plates (blue or white).

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