Assignment 4 - 2017
- For both the targeted and the shotgun cloning methods, draw plasmid maps of all possible 2 piece recombinant molecules.
- Indicate the total size of each recombinant and the source and size of the 2 fragments.
- Indicate the relevant restriction enzyme sites (abbreviations are fine, just give us a key), the origins of replication, the antibiotic resistance genes, and the location of Gene A, if present.
- For each of the above recombinants indicate if the bacteria that take it up will
- be able to replicate the plasmid (indicate as 'replicatable')
- grow on ampicillin-containing plates (ampR) and
- if the resulting colonies will be blue or white on x-gal containing plates (blue or white).