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The 2015 lab manual will be posted sometime in August.


Re: Lab manual? by Lauri_BioLauri_Bio, 15 Jun 2015 17:57
Lab manual?
Lauri_BioLauri_Bio 15 Jun 2015 17:57
in discussion Lab Discussions / Questions » Lab manual?

When will the 2015 version of the protocol manual be available?


Lab manual? by Lauri_BioLauri_Bio, 15 Jun 2015 17:57

Hi Prasuna,
Sorry I missed this post early. I have fixed the link. The same info is available as an appendix - hope you managed to find it.


Blue-white screening by Lauri_BioLauri_Bio, 01 Oct 2012 03:50

Hello Lauri,

On the Project overview page, in section V, when I click on the blue/white screening link, Wikidot is saying that such a page doesn't exist….Is it possible to fix that link?

Thank you,

Blue/white screening by Prasuna KunasaniPrasuna Kunasani, 18 Sep 2012 00:35

Make sure you have planned your digests and worked out how to make the master mixes. You won't have time to do this in lab. If you are having trouble, please see Lauri at some point BEFORE the lab.


Before you come to lab by Lauri_BioLauri_Bio, 18 Oct 2011 23:29

Assignment 2 is a draft of the materials and methods for your entire lab report - not just this weeks lab. They should be written as you would write them for the actual lab report (no more than 2 pages). Please read these tips.


Materials and Methods by Lauri_BioLauri_Bio, 18 Oct 2011 23:26

There is an error in the Weekly Guides. Ethidium bromide final concentration in gels should be 0.1 µg/ml, not 0.5 µg/ml. This has been corrected in the Guides as of Sept. 27, 2011.


Some general pointers on the notebooks from week 1.

Make sure you are clear on source of reagents. This week the plasmid isolation solutions all came with the Fermentas GeneJet kit. Other weeks they may come from a different kit, or be made in house.

Notes need to be complete - but do try and be brief - point form is great - avoid repeating the same info over and over (for example if all spins were at top speed you can make a note at the start). Use accepted SI unit abbreviations and use symbols for numbers rather than spelling out ; e.g. 1 min NOT one minute, 60 s, not 60 seconds or sixty seconds. Not only will this save time and ink, but it is the correct way to represent numbers in science.


Lab notebooks by Lauri_BioLauri_Bio, 22 Sep 2011 16:48

I have finished marking the Notebooks for Tue and Wed groups, I should finish Thur later this afternoon. You can pick them up from my office (LB414.4). I should be in my office or the lab for most of today.



Notebooks - week 1 by Lauri_BioLauri_Bio, 19 Sep 2011 15:30
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