Data 2016

Lab photos
Datasheet

Notes

I have closed off the sheets to editing (except by me) as of Monday, Nov 7th.
I have made some fixes where data was misinterpreted by various groups.
EDITED AGAIN on Nov 8th - changes highlighted in yellow (on the Monday sheet).

Analysis

  • Please use all groups data (all of Monday + all of Tuesday).
  • I found it easiest to download this as an Excel file before messing with it.
  • The two most important columns are the cloning strategy (S or T) and the identification of the recombinant.
  • Because the identification has merged rows, you can't easily auto sort, it works better to duplicate the sheets, and on one sheet delete the Ts and on the other delete the Ss.
  • Use Excel Countif to easily add up the number of recombinants observed for each type.
  • By my count, we determined the identity of shotgun recombinants and targeted recombinants (I did not include "can't tell" in these counts).
  • One of our goals was to analyze and compare the two approaches to subcloning. Part of this analysis should include how many different recombinants were produced by each method and how common they were as this might be important for future applications. For example, for the shotgun method, we predicted there could be up to six different 2 piece recombinants that would produce white colonies. If some of the predicted recombinants were not observed, then this must be considered when evaluating the method. Along the same lines, we did not expect to see any 4 piece recombinants, but we identified 11 in the shotgun recombinants.
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